Gamete binning: chromosome-level and haplotype-resolved genome assembly enabled by high-throughput single-cell sequencing of gamete genomes

Use of high throughput sequencing to observe genome dynamics at a single cell level.

With the development of high throughput sequencing technology, it becomes possible to directly analyze mutation distribution in a genome-wide fashion, dissociating mutation rate measurements from the traditional underlying assumptions. Here, we sequenced several genomes of Escherichia coli from colonies obtained after chemical mutagenesis and observed a strikingly nonrandom distribution of the ...

Haplotype resolved genomes

How Single Molecule Real-Time Sequencing and Haplotype Phasing Have Enabled Reference-Grade Diploid Genome Assembly of Wine Grapes

High throughput sequencing technologies — an algorithm for genome assembly

s 44 2010

Gamete preservation.

With the increase in survivorship following cancer for women in their reproductive years, as well as an increase in survivorship with childhood cancers, there is a demand for perfecting current fertility preservation methods and generating new options for patients who are unable to pursue the conventional course of fertility treatments. Cryopreservation using a slow-cooling method for embryos i...